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英文名称：PSP Spin Stool DNA Plus Kit
The PSP Spin Stool DNA Plus Kit is an integrated system for collection, transportation and storage of stool samples and subsequent DNA purification. Transportation of the stabilized DNA can be carried out in the DNA Stabilizer solution without refrigeration at ambient temperature. The purification kit is designed for DNA isolation from pathogenic microorganisms and from the host organism.
- All-in-one product - from collection to isolation: pre-filled collection tubes with Stool DNA stabilizer
- Samples are stable at room temperature for 3 months for easy transport and storage
- Inactivation of DNases and preservation of the microorganism titre
- For In Vitro Diagnostic Use (CE-IVD)*
a spoon of stool sample
(about 1 g) collected in
8 ml of Stool Stabilizer
up to 50 μg/ 1.4 ml of
stabilized stool sample
about 60 min
For the DNA extraction process a small aliquot of the total volume is used, and the residual sample can be stored in the DNA stabilization buffer at -20°C for further extractions. The PSP Spin Stool DNA Plus Kit uses a precleaning step, optimized buffer and essential washing conditions to remove potent inhibitors very efficiently. The purified DNA is ready to use for subsequent downstream applications:
• PCR applications
• genetic typing
• pathogen typing
• mutation analysis
• ancestry research
• feed and food determination
*) Compliance with EU Directive 98/79/EC on in vitro medical devices. Products which are CE-marked according to the IVD-Directive can be used for diagnostic applications in countries where this directive is recognized.
PSP Spin Stool DNA Plus Kit
PSP Spin Stool DNA Plus Kit
Stool Collection Tubes
with Stool DNA Stabilizer
50 prefilled tubes
Stool Collection Tubes
with Stool DNA Stabilizer
Stool DNA Stabilizer
1. High DNA yields from stool samples
Total DNA was isolated using the PSP Spin Stool DNA Plus Kit and a competitor kit Q from 20 different stool samples according to the standard protocols. The total DNA concentration was measured on a NanoDrop (figure A) and the respective microorganism DNA content (from Bifidobacterium sp., Escherichia coli, Faecalibacterium prausnitzii, Lactobacillus sp.) was analyzed and measured via quantitative real-time PCR (figure B).
Data kindly provided by S.C. Bischoff, University Hohenheim, Stuttgart, Germany
2. Detection of Helicobacter pylori in human stool samples
Amplification of different target sequences of extracted bacterial DNA (H. pylori) using the PSP Spin Stool DNA Plus Kit from human stool samples stored at room temperature (without refrigeration) for 3 days with Stool Stabilizer in a Stool Collection Tube.
3. Detection of Mycobacterium avium ssp. paratuberculosis in stool samples from cattle
DNA was isolated from clinical and spiked stool samples from cattle using the PSP Spin Stool DNA Plus Kit. The isolated DNA was used for PCR amplification of the M. avium ssp. paratuberculosis target sequence ISMav2 (using the Bactotype Amplification Kit Mycobacterium paratuberculosis) with a sensitivitiy of 102 cells/g stool (6-fold repeat).
Data kindly provided by Dr. F. Kloep, Biotype AG.
User manual download
Selected referencesA comparison of rumen microbial profiles in dairy cows as retrieved by 454 Roche and Ion Torrent (PGM) sequencing platforms.
Indugu N, Bittinger K, Kumar S, Vecchiarelli B, Pitta D
PeerJ. 2016 Feb 4;4:e1599
An Integrated Metabolomic and Microbiome Analysis Identified Specific Gut Microbiota Associated with Fecal Cholesterol and Coprostanol in Clostridium difficile Infection.
Antharam VC, McEwen DC, Garrett TJ, Dossey AT, Li EC, Kozlov AN, Mesbah Z, Wang GP
PLoS One. 2016 Feb 12;11(2):e0148824
Bidirectional interactions between indomethacin and the murine intestinal microbiota.
Liang X, Bittinger K, Li X, Abernethy DR, Bushman FD, FitzGerald GA
Elife. 2015 Dec 23;4:e08973
The stool microbiota of insulin resistant women with recent gestational diabetes, a high risk group for type 2 diabetes.
Fugmann M, Breier M, Rottenkolber M, Banning F, Ferrari U, Sacco V, Grallert H, Parhofer KG, Seissler J, Clavel T, Lechner A
Sci Rep. 2015 Aug 17;5:13212
Rhythmicity of the intestinal microbiota is regulated by gender and the host circadian clock.
Liang X, Bushman FD, FitzGerald GA
Proc Natl Acad Sci U S A. 2015 Aug 18;112(33):10479-84
Effects of surgical and dietary weight loss therapy for obesity on gut microbiota composition and nutrient absorption.
Damms-Machado A, Mitra S, Schollenberger AE, Kramer KM, Meile T, Königsrainer A, Huson DH, Bischoff SC
Biomed Res Int. 2015;2015:806248
Seeking the source of Pseudomonas aeruginosa infections in a recently opened hospital: an observational study using whole-genome sequencing.
Quick J, Cumley N, Wearn CM, Niebel M, Constantinidou C, Thomas CM, Pallen MJ, Moiemen NS, Bamford A, Oppenheim B, Loman NJ
BMJ Open. 2014 Nov 4;4(11):e006278
Temporal dynamics in the ruminal microbiome of dairy cows during the transition period.
Pitta DW, Kumar S, Vecchiarelli B, Shirley DJ, Bittinger K, Baker LD, Ferguson JD, Thomsen N
J Anim Sci. 2014 Sep;92(9):4014-22
Reagent contamination can critically impact sequence-based microbiome analyses.
Susannah Salter, Michael J Cox, Elena M Turek, Szymon T Calus, William O Cookson, Miriam F Moffatt, Paul Turner, Julian Parkhill, Nick Loman, Alan W Walker
Fecal microbial composition of ulcerative colitis and Crohn's disease patients in remission and subsequent exacerbation.
Wills ES, Jonkers DM, Savelkoul PH, Masclee AA, Pierik MJ, Penders J
PLoS One. 2014 Mar 7;9(3):e90981
Life-threatening angioedema of the tongue: the detection of the RNA of B henselae in the saliva of a male patient and his dog as well as of the DNA of three Bartonella species in the blood of the patient.
Lösch B, Wank R
BMJ Case Rep. 2014 Mar 20;2014
Analysis of the intestinal microbiota using SOLiD 16S rRNA gene sequencing and SOLiD shotgun sequencing.
Mitra S, Förster-Fromme K, Damms-Machado A, Scheurenbrand T, Biskup S, Huson DH, Bischoff SC
BMC Genomics. 2013;14 Suppl 5:S16
Chronic kidney disease alters intestinal microbial flora.
Vaziri ND, Wong J, Pahl M, Piceno YM, Yuan J, DeSantis TZ, Ni Z, Nguyen TH, Andersen GL
Kidney Int. 2013 Feb;83(2):308-15
Differentiation of Entamoeba histolytica/Entamoeba dispar by the polymerase chain reaction in stool samples of patients with gastrointestinal symptoms in the Sanliurfa Province.
Zeyrek FY, Turgay N, Unver A, Ustün S, Akarca U, Töz S
Turkiye Parazitol Derg. 2013;37(3):174-8
Structural modulation of gut microbiota in life-long calorie-restricted mice.
Zhang C, Li S, Yang L, Huang P, Li W, Wang S, Zhao G, Zhang M, Pang X, Yan Z, Liu Y, Zhao L
Nat Commun. 2013;4:2163
A sensitive and specific multiplex PCR approach for sex identification of ursine and tremarctine bears suitable for non-invasive samples.
Bidon T, Frosch C, Eiken HG, Kutschera VE, Hagen SB, Aarnes SG, Fain SR, Janke A, Hailer F
Mol Ecol Resour. 2013 May;13(3):362-8
Succession in the gut microbiome following antibiotic and antibody therapies for Clostridium difficile.
Peterfreund GL, Vandivier LE, Sinha R, Marozsan AJ, Olson WC, Zhu J, Bushman FD
PLoS One. 2012;7(10):e46966
A tool kit for quantifying eukaryotic rRNA gene sequences from human microbiome samples.
Dollive S, Peterfreund GL, Sherrill-Mix S, Bittinger K, Sinha R, Hoffmann C, Nabel CS, Hill DA, Artis D, Bachman MA, Custers-Allen R, Grunberg S, Wu GD, Lewis JD, Bushman FD
Genome Biol. 2012 Jul 3;13(7):R60. [Epub ahead of print]
Limited gene flow among brown bear populations in far Northern Europe? Genetic analysis of the east-west border population in the Pasvik Valley.
Schregel J, Kopatz A, Hagen SB, Brøseth H, Smith ME, Wikan S, Wartiainen I, Aspholm PE, Aspi J, Swenson JE, Makarova O, Polikarpova N, Schneider M, Knappskog PM, Ruokonen M, Kojola I, Tirronen KF, Danilov PI, Eiken HG
Mol Ecol. 2012 Jul;21(14):3474-88
Innate lymphoid cells promote anatomical containment of lymphoid-resident commensal bacteria.
Sonnenberg GF, Monticelli LA, Alenghat T, Fung TC, Hutnick NA, Kunisawa J, Shibata N, Grunberg S, Sinha R, Zahm AM, Tardif MR, Sathaliyawala T, Kubota M, Farber DL, Collman RG, Shaked A, Fouser LA, Weiner DB, Tessier PA, Friedman JR, Kiyono H, Bushman FD, Chang KM, Artis D
Science. 2012 Jun 8;336(6086):1321-5
The development of a loop-mediated isothermal amplification method (LAMP) for Echinococcus granulosus [corrected] coprodetection.
Salant H, Abbasi I, Hamburger J
Am J Trop Med Hyg. 2012 Nov;87(5):883-7
Sampling and pyrosequencing methods for characterizing bacterial communities in the human gut using 16S sequence tags.
Wu GD, Lewis JD, Hoffmann C, Chen YY, Knight R, Bittinger K, Hwang J, Chen J, Berkowsky R, Nessel L, Li H, Bushman FD
BMC Microbiol. 2010 Jul 30;10:206