Protease Inhibitor Cocktail (EDTA-Free, 100X in DMSO)
Endogenous proteins are produced and removed in a balanced state, so their cellular levels are generally stable under stable environmental conditions. However, protein production is greatly halted and degradation is enhanced when cells are studied in vitro. To prevent the degradation of proteins under such conditions, one can utilize a cocktail of small molecule inhibitors to block the action of proteases. The cocktail functions to inhibit proteases that would degrade either phosphorylated or non-phosphorylated protein substrates.
MedChemExpress Protease Inhibitor Cocktail (EDTA free, 100X in DMSO) is a blend of 6 pan-protease inhibitors for protection of protein integrity. This mixture contains individual components, including AEBSF at 104 mM, Aprotinin at 80 μM, Bestatin at 5 mM, E-64 at 1.5 mM, Leupeptin at 2 mM and Pepstatin A at 1.5 mM. Each component has specific inhibitory properties. AEBSF and Aprotinin act to inhibit serine proteases, including trypsin, chymotrypsin, and plasmin amongst others. Bestatin inhibits aminpeptidases. E-64 acts against cystein proteases. Leupeptin acts against both serine and cystein proteases. Pepstatin A inhibits acid proteases.
1)This product can be applied in Western Blot analysis, Co-IP, pull-down, IF, IHC, kinase assay and etc.
2) Thaw at room temperature, add at 1:100 (v/v) dilution to 1 solution samples (such as cell lysates or tissue extracts), before assaying. One tube is recommended for inhibition of proteases present in cell.
3) Extracts of up to 20 g wet weight.
Protease Inhibitor Cocktail (EDTA free, 100X) can be stored at -20°C for 12 months.
1) DMSO solution thaws slowly in low temperature. Recommend to warm up at 25°C.
2) Not all lysates contain the same levels of endogenous enzymes, and it may be necessary to adjust the volume of cocktail required.
6.Precautions and Disclaimer
This product is for R&D use only, not for drug, household, or other uses. Please consult the Material Safety Data Sheet for information regarding hazards and safe handling practices.